This study was designed to study on major components of various Bee Venom(Bee Venom by electrical stimulation in Korea; K-BV I , Bee Venom by Microwave stimulation in Korea; K-BV II, 0.5㎎/㎖, Fu Yu Pharmaceutical Factory, China; C-BV, 1㎎/㎖, Monmouth Pain Institute, Inc., U.S.A.; A-BV) using HPLC(High performance liquid chromatography).
The results were summarized as follows :
1. HPLC method is useful for analysis of Bee Venom when solution rate is above 1:4000.
2. Analysis of Apamin using HPLC, the Retention time was 8.7min, and standard measurement curve was a function of y=4E+06x+21245.
3. Analysis of Melittin using HPLC, the Retention time was 29.0 min, and standard measurement curve was a function of y=4E+06x+23015.
4. Concentration of Melittin was about 297times than Apamin in K-BV I , and about 329times in K-BV II at same 1:500 solution rate, abnormally about 12 times in C-BV at 1:4000 solution rate.
5. Chinese Bee Venom using HPLC, the point from 5 to 7min(Retention time) showed a big extraordinary peak.
These data from the study can be applied to establish the standard measurement of Bee Venom and prevent pure bee venom from mixing of another components. I think it is desirable to study more about safety of Bee Venom as time goes by.
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